Small molecule inhibitors of polynucleotide kinase/phosphatase, poly(adp-ribose) polymerase and uses thereof

ABSTRACT

The present invention generally relates to use of compounds and compositions as a chemosensitizers and/or radiosensitizers and/or inhibitors of PNKP phosphatase activity. The present invention provides pharmaceutical combinations and/or a pharmaceutically acceptable salt thereof, kits containing such compounds and/composition and methods of using such compounds and/or compositions.

RELATED APPLICATIONS

This application is a continuation of application Ser. No. 13/375,876,filed Feb. 20, 2012, which is the U.S. national phase of InternationalApplication No. PCT/CA2010/000846, filed Jun. 4, 2010, which designatedthe U.S. and claims the benefit of priority to U.S. ProvisionalApplication Ser. No. 61/184,013, filed Jun. 4, 2009, and to U.S.Provisional Application Ser. No. 61/263,711, filed Nov. 23, 2009, all ofwhich are hereby incorporated in their entirety including all tables,figures, and claims.

SEQUENCE LISTING

The instant application contains a Sequence Listing which has beensubmitted in ASCII format via EFS-Web and is hereby incorporated byreference in its entirety. Said ASCII copy, created on Jul. 10, 2015, isnamed PRHM005CT_SeqListing.txt and is 1 kilobyte in size.

FIELD OF THE INVENTION

The field of the invention generally relates to inhibitors ofpolynucleotide kinase/phosphatase and poly(ADP-ribose) polymerase, andtheir compounds, compositions, methods and kits and uses thereof.

BACKGROUND OF THE INVENTION

Radiation and systemic chemotherapy are important therapeutic modalitiesfor the treatment of cancer. Nuclear DNA is considered to be a majorcellular target responsible for the cytotoxicity of ionizing radiationand many conventional antineoplastic drugs. As a consequence, the levelsof DNA damage and its repair are likely to influence cell survival andaffect clinical outcome (1).

The manipulation of DNA repair systems has recently become the focus ofconsiderable interest as a means of enhancing the efficacy of radio- andchemotherapy. Particular emphasis has been placed on single anddouble-strand break repair pathways (2). Small molecule inhibitors havenow been developed that target enzymes such as poly(ADP-ribose)polymerase (PARP) and apurinic/apyrimidinic endonuclease (APE1), whichare involved in the repair of damaged bases and single-strand breaksinduced by many agents including ionizing radiation and alkylatingagents (1, 3, 4); tyrosyl DNA-phosphodiesterase (Tdp1), which isrequired for the repair of strand breaks introduced by topoisomerase 1inhibitors such as camptothecin and irinotecan (5); and ATM and DNA-PK,which regulate the response to DNA double-strand breaks (6, 7).Inhibitors of PARP are now in clinical trial (8).

Ionizing radiation and other genotoxic agents often generate strandbreaks with incompatible termini that must be processed in order forsingle and double-strand break repair pathways to complete the repair.Among the frequently observed termini are 3′-phosphate andphosphoglycolate and 5′-hydroxyl groups (9, 10). These lesions create abarrier for DNA polymerases and ligases to replace missing bases andseal the breaks because these enzymes have a strict requirement for thepresence of a 3′-hydroxyl group and in addition DNA ligases require a5′-phosphate group (11, 12).

A major enzyme responsible for the phosphorylation of 5′-hydroxyltermini and dephosphorylation of 3′-phosphate termini in human cells ispolynucleotide kinase/phosphatase (hPNKP) (13, 14). In the single-strandbreak (SSB) repair pathway hPNKP acts in concert with XRCC1, DNApolymerase β and DNA ligase III (15-17). PNKP-mediated DNAend-processing at double-strand breaks is a component of thenonhomologous end-joining (NHEJ) pathway and is dependent on DNA-PKcsand XRCC4 (18-20). In addition to its role in the repair of strandbreaks produced directly by genotoxic agents, hPNKP has been implicatedin the repair of strand breaks produced by enzymatic processes,including strand breaks introduced by the βδ-AP lyase activity of DNAglycosylases such as NEIL1 and NEIL2 (21, 22), which generate3′-phosphate termini. Similarly, hPNKP is required to process terminigenerated by the topoisomerase I inhibitor camptothecin (23). Treatmentwith camptothecin stalls topoisomerase I while it is covalently attachedto a 3′-phosphate group in the course of its nicking-resealing activity.The stalled enzyme can be cleaved from the DNA by Tdp1 leaving a strandbreak with 3′-phosphate and 5′-hydroxyl termini, which necessitates thesubsequent action of PNKP. Down-regulation of hPNKP by RNAi sensitizedcells to a variety of genotoxic agents including ionizing radiation,camptothecin, methyl methanesulfonate and hydrogen peroxide (24). Itremains to be determined which of hPNKP's activities, 5′-kinase or3′-phosphatase (or both), is responsible for sensitization to eachagent. The two activities are independent with separate DNA bindingdomains (25), but the phosphatase reaction appears to proceed ahead ofthe kinase reaction (26).

Synthetic lethality occurs when a combination of two protein knockoutsis lethal, however the corresponding single mutations are viable. Theoriginal concept of synthetic lethality as it relates to DNA repair wasdiscovered in 2005. The Ashworth and Helleday groups published twopapers back to back in Nature, outlining synthetic lethality betweenBRCA−/− cells and inhibition of poly(ADP-ribose) polymerase (PARP).

It is, therefore, desirable to provide inhibitors of DNA repair proteinssuch as polynucleotide kinase/phosphatase and poly(ADP-ribose)polymerase, and their compounds, compositions, methods and kits and usesthereof.

This background information is provided for the purpose of making knowninformation believed by the applicant to be of possible relevance to thepresent invention. No admission is necessarily intended, nor should itbe construed, that any of the preceding information constitutes priorart against the present invention.

SUMMARY OF THE INVENTION

An object of the present invention is to provide a compound(s),composition(s), method(s) and/or kit for increasing the sensitivity of acell(s) and/or tumour(s) to chemotherapeutic agents and/or ionizingradiation.

In accordance with one aspect of the rpesent invention, there isprovided, a compound or pharmaceutically acceptable salt thereof forincreasing the sensitivity of a cancerous cell of a patient to achemotherapeutic agent or radiation therapy, said compound comprising:2-(1-hydroxyundecyl)-1-(4-nitrophenylamino)-6-phenyl-6,7a-dihydro-1H-pyrrolo[3,4-b]pyridine-5,7(2H,4aH)-dione (A12B4C3),2-(hydroxy(phenyl)methyl)-1-(4-nitrophenylamino)-6-phenyl-6,7a-dihydro-1H-pyrrolo[3,4-b]pyridine-5,7(2H,4aH)-dione(A1B4C3),2-(hydroxy(3,4,5-trimethoxyphenyl)methyl)-1-(4-nitrophenylamino)-6-phenyl-6,7a-dihydro-1H-pyrrolo[3,4-b]pyridine-5,7(2H,4aH)-dione(A6B4C3), tert-butyl2-(1-hydroxy-2,2-diphenylethyl)-6-methyl-5,7-dioxo-2,4a,5,6,7,7a-hexahydro-1H-pyrrolo[3,4-b]pyridin-1-ylcarbamate(A26B11C2), or2-(hydroxy(thiophen-2-yl)methyl)-6-methyl-1-(phenylamino)-6,7a-dihydro-1H-pyrrolo[3,4-b]pyridine-5,7(2H,4aH)-dione(A39B1C2).

In accordance with another aspect of the present invention, there isprovided a method of chemosensitizing or radiosensitizing a cancerouscell in a mammal in need of chemotherapy or radiation therapy,comprising: administering to said mammal a compound or pharmaceuticallyacceptable salt thereof selected from A12B4C3, A1B4C3, A6B4C3, A26B11C2or A39B1C2.

In accordance with another aspect of the present invention, there isprovided a method of inhibiting the phosphatase activity of PNK,comprising: contacting a cell with a compound or pharmaceuticallyacceptable salt thereof selected from A12B4C3, A1B4C3, A6B4C3, A26B11C2or A39B1C2.

In accordance with another aspect of the present invention, there isprovided an improved method for radiation therapy of a patient with aneoplasm employing a radiation sensitizer, wherein the improvementcomprises treating said patient with an effective amount of a compoundor pharmaceutically acceptable salt thereof selected from A12B4C3,A1B4C3, A6B4C3, A26B11C2 or A39B1C2 as the radiation sensitizer.

In accordance with another aspect of the present invention, there isprovided an improved method for chemotherapy therapy of a patient with aneoplasm employing a chemosensitizer, wherein the improvement comprisestreating said patient with an effective amount of a compound orpharmaceutically acceptable salt thereof selected from A12B4C3, A1B4C3,A6B4C3, A26B11C2 or A39B1C2 as the chemosensitizer.

In another aspect of the present invention, there is provided a kit forincreasing the sensitivity of a cancerous cell to a chemotherapeuticagent or radiation therapy said kit comprising: a compound orpharmaceutically acceptable salt thereof selected from A12B4C3, A1B4C3,A6B4C3, A26B11C2 or A39B1C; and instructions for the use thereof.

In another aspect of the present invention, there is provided a methodof treating a subject suffering from a disorder associated with a defectin DNA polymerase β, comprising administering to said subject aninhibitor of PNKP.

In another aspect of the present invention, there is provided apharmaceutical composition comprising: a first amount of a topoisomeraseI inhibitor and a second amount of a PNKP inhibitor, and apharmaceutically acceptable carrier.

In another aspect of the present invention, there is provided acombination comprising a topoisomerase I inhibitor and a PNKP inhibitor

In accordance with another aspect of the present invention, there isprovided a compound(s), composition(s), method(s) and/or kit forinhibiting PNKP phosphatase activity.

In accordance with another aspect of the present invention, there isprovided a compound for increasing the sensitivity of a cell and/ortumour to a chemotherapeutic agent and/or ionizing radiation, thecompound comprising:

2-(1-hydroxyundecyl)-1-(4-nitrophenylamino)-6-phenyl-6,7a-dihydro-1H-pyrrolo[3,4-b]pyridine-5,7(2H,4aH)-dione(A12B4C3),

2-(hydroxy(phenyl)methyl)-1-(4-nitrophenylamino)-6-phenyl-6,7a-dihydro-1H-pyrrolo[3,4-b]pyridine-5,7(2H,4aH)-dione(A1B4C3),

2-(hydroxy(3,4,5-trimethoxyphenyl)methyl)-1-(4-nitrophenylamino)-6-phenyl-6,7a-dihydro-1H-pyrrolo[3,4-b]pyridine-5,7(2H,4aH)-dione (A6B4C3),

tert-butyl2-(1-hydroxy-2,2-diphenylethyl)-6-methyl-5,7-dioxo-2,4a,5,6,7,7a-hexahydro-1H-pyrrolo[3,4-b]pyridin-1-ylcarbamate(A26B11C2), or

2-(hydroxy(thiophen-2-yl)methyl)-6-methyl-1-(phenylamino)-6,7a-dihydro-1H-pyrrolo[3,4-b]pyridine-5,7(2H,4aH)-dione(A39B1C2).

In accordance with one aspect of the present invention there is provideda chemosensitization and/or radiosensitization method to treat a cell invitro and/or in vivo comprising administering to said cell a compoundcomprising A12B4C3, A1B4C3, A6B4C3, A26B11C2 or A39B1C2.

In a specific example the cell is A549 or MDA-MB-231.

In another aspect of the present invention, there is provided a methodof radiosensitizing tumor cells in a mammal in need of radiationtherapy, comprising administering to said mammal a compound selectedfrom A12B4C3, A1B4C3, A6B4C3, A26B11C2 or A39B1C2

In one aspect of the present invention there is provided a use of acompound selected from A12B4C3, A1B4C3, A6B4C3, A26B11C2 or A39B1C2 inthe preparation of a pharmaceutical composition for use as aradiosensitizer.

In one aspect of the present invention there is provided a use of acompound selected from A12B4C3, A1B4C3, A6B4C3, A26B11C2 or A39B1C2 inthe preparation of a pharmaceutical composition for use as achemosensitizer.

In one aspect of the present invention there is provided a use of acompound selected from A12B4C3, A1B4C3, A6B4C3, A26B11C2 or A39B1C2 inthe preparation of a pharmaceutical composition for use as an inhibitorof the phosphatase activity of PNKP.

In a specific example, the phosphatase activity of PNKP is selected fromhuman PNKP or mouse PNKP.

In accordance with another aspect of the present invention there isprovided PNKP phosphatase inhibitor selected from A12B4C3, A1B4C3,A6B4C3, A26B11C2 or A39B1C2 to prepare a pharmaceutical composition toprevent or treat a cancer in a mammal, wherein the pharmaceuticalcomposition is intended for administration in combination with achemotherapeutic agent and/or ionizing radiation used in a treatment ofa cancer.

In a specific example, the chemotherapeutic agent is a topoisomerase Iinhibitor. In a specific example, the topoisomerase inhibitor isCamptothecin.

In another specific example, the ionizing radiation is γ-radiation. Inone example, the ionizing radiation is X-rays generated by a linearaccelerator (Linac).

In accordance with another aspect of the present invention, there isprovided an improved method for radiation therapy of a patient with aneoplasm employing a radiation sensitizer, wherein the improvementcomprises treating said patient with an effective amount of a compoundselected from A12B4C3, A1B4C3, A6B4C3, A26B11C2 or A39B1C2 as theradiation sensitizer.

In accordance with another aspect of the present invention, there isprovided an improved method for chemotherapy therapy of a patient with aneoplasm employing a chemosensitizer, wherein the improvement comprisestreating said patient with an effective amount of a compound selectedfrom A12B4C3, A1B4C3, A6B4C3, A26B11C2 or A39B1C2 as thechemosensitizer.

In accordance with one aspect of the present invention, there isprovided method of treating a mammal diagnosed with cancer, said methodcomprising administering to said mammal a therapeutically effectiveamount of a pharmacological composition comprising a compound selectedfrom A12B4C3, A1B4C3, A6B4C3, A26B11C2 or A39B1C wherein saidcomposition contacts a cancer cell or tumour in said mammal, therebymaking said cancer cell or tumour more susceptible to the effects ofchemotherapy and/or ionizing radiation.

In a specific example, the chemotherapeutic agent is a topoisomerase Iinhibitor. In a specific example, the topoisomerase inhibitor isCamptothecin.

In another specific example, the ionizing radiation is y-radiation. Inone example, the ionizing radiation is X-rays generated by a linearaccelerator (Linac).

In accordance with another aspect of the present invention there isprovided a kit for increasing the sensitivity of a cell(s) and/ortumour(s) to a chemotherapeutic agent and/or ionizing radiation or forinhibiting the phosphatase activity of PNKP, said kit comprising:

-   -   (i) a compound selected from A12B4C3, A1B4C3, A6B4C3, A26B11C2        or A39B1C; and    -   (ii) instructions for the use thereof.

In a specific example, the chemotherapeutic agent is a topoisomerase Iinhibitor. In a specific example, the topoisomerase inhibitor isCamptothecin.

In another specific example, the ionizing radiation is γ-radiation. Inone example, the ionizing radiation is X-rays generated by a linearaccelerator (Linac).

In another specific example, the phosphatase activity PNKP is humanPNKP, or mouse PNKP.

In accordance with another aspect of the present invention, there isprovided a method for the treatment of a subject suffering from adisorder, such as cancer, associated with a defect in DNA polymerase β,comprising administering to said subject an inhibitor of PNKP. In oneexample the inhibitor of PNKP is A12B4C3 (also referred to as H5 herein,and in the Figures).

In accordance with another aspect of the present invention, there isprovided a method for the treatment of a subject suffering from adisorder, such as cancer, associated with a defect in PNKP or DNA-PK,comprising administering to said subject an inhibitor of PARP In oneexample the inhibitor of PARP is DPQ.

BRIEF DESCRIPTION OF THE DRAWINGS

Embodiments of the present invention will now be described, by way ofexample only, with reference to the attached Figures, wherein:

FIG. 1A depicts a typical phosphatase standard curve for thefluorescence quenching-based phosphatase assay. Readings were taken forsolutions consisting of 0, 25, 50 and 100% phosphorylated oligos(r=0.99). A fresh standard curve was generated each time the screeningassay was performed. FIG. 1B depicts loss of fluorescence quenchingresulting from increased removal of the 3′-phosphate group from thesubstrate with increasing quantity of hPNKP in the reaction. The dataare combined from 3 independent determinations±S.E.M. FIG. 1C depictsresults of the screening assay for eight of the small molecules tested.Compounds A4B8C2, A28B3C1 and A24B12C3 failed to show any quenching ofthe sensor molecule as a result of hPNKP inhibition, while A12B4C3,A1B4C3, A6B4C3, A26B11C2 and A39B1C2 all displayed marked inhibition ofsubstrate dephosphorylation. The data are combined from 3 independentdeterminations±S.E.M;

FIG. 2 depicts the chemical structures and names of the compounds foundto inhibit hPNKP phosphatase activity;

FIG. 3 depicts inhibition of hPNKP 3′-phosphatase activity usingconventional radio-gel assay. A 20mer oligonucleotide with a3′-phosphate was labelled at the 5′-terminus with [γ-³²P]ATP (*p20p),which is acted on by hPNKP, resulting in the removal of the3′-phosphate. This produces *p20 which has a slower mobility in the gel.Addition of the small molecule inhibitors reduces the conversion of*p20p to *p20;

FIGS. 4A-4C depict the measurement of 3′-phosphatase activity by thePiColorlock Gold assay. FIG. 4A depicts a typical standard curvegenerated by mixing specific ratios of 3′-phosphorylated andnon-phosphorylated 20mer oligonucleotides (final concentration ofoligonucleotide=100 μM) in 4 separate tubes, which were then treatedwith hPNKP for 30 minutes at 37° C., conditions that lead to complete3′-dephosphorylation of the oligonucleotide. FIG. 4B depictsconcentration dependence of phosphatase inhibition by the fiveidentified compounds. The data are drawn from 3 independent assays.Error bars indicate the S.E.M. FIG. 4C depicts determination of the IC₅₀values of the two most potent inhibitory compounds derived from 3independent assays. The curves were fitted using GraphPad Prism software(GraphPad Software Inc., La Jolla, Calif.);

FIGS. 5A-5E depict specificity of inhibition by A12B4C3. FIG. 5A depictsinhibition of T4 PNK and fission yeast PNKP by 50 μM A12B4C3 measured bythe PiColorLock assay. FIG. 5B depicts a comparison of dose dependenceof inhibition of mouse and human PNKPs by A12B4C3 measured by thePiColorLock assay. FIG. 5C depicts a comparison of the 3′-DNAphosphatase activities of hPNKP and aprataxin (APTX) in the absence andpresence of A12B4C3. The oligonucleotide substrate was incubated withequal quantities of the two enzymes that were purified on the same day.FIG. 5D depicts the influence of A12B4C3 on protein phosphatases, whichwas examined as described in Material and Methods. No inhibition byA12B4C3 of PP-1 or calcineurin (CaN) was observed. In comparisonmicrocystin LR (12 nM) inhibited PP-1. FIG. 5E depicts dose-dependentinhibition of hPNKP DNA kinase activity by A12B4C3 measured by thetransfer of radiolabeled phosphate from [γ-³²P]ATP as described inMaterials and Methods. Data for each figure was compiled from 3independent assays for each activity measured. The error bars show theS.E.M.;

FIGS. 6A-6C depict radiosensitization by A12B4C3. FIG. 6A depictscytotoxicity of A12B4C3 alone measured by 72-hour exposure of A549 lungcancer cells and MDA-MB-231 cells to increasing concentrations of thecompound and determination of cell proliferation as described inMaterials and Methods. The data are drawn from 3 independentdeterminations±S.E.M. FIG. 6B depicts the influence of A12B4C3 on theradiosensitivity of wild-type A549 cells and PNKP-deficient cells(A549δPNKP). Cells were exposed to 1 μM A12B4C3 two hours prior toirradiation and then maintained in the same media for a further 24hours. The media was then replaced with fresh media without the drug.Cytotoxicity was determined by the colony forming assay as described inMaterials and Methods. The survival curves (±S.E.M.) are based on 4independent sets of determinations. FIG. 6C depicts the influence ofA12B4C3 on the radiosensitivity of wild type MDA-MB-231 cells andPNKP-depleted MDA-MB-231 cells (MDA-MB-231δPNKP) using identicalconditions to those described in FIG. 6B. The survival curves (±S.E.M.)are based on 5 independent sets of determinations;

FIG. 7 is a graph depicting cell survival for A549 and A549δPNKP cellswith Camptothecin+/−A12B4C3;

FIG. 8 is a graph depicting synthetic lethality of DNA polymerase β andPNKP. A) there is an inverse relationship between the concentration ofPNKP inhibitor A12B4C3 and cell survival, indicating that co-disruptionof DNA polymerase β and PNKP is synthetically lethal. PolβDN=A549 cellsstably expressing a dominant negative form of DNA polymerase β. Thisvector encodes only the DNA binding domain of DNA polymerase β and notthe catalytic domain, thereby acting as a dominant negative towardsendogenous DNA polymerase β. A12B4C3=PNKP inhibitor andA549-LZ=A549-vector only cells and were used as our negative control.A549δPNKP cells are shown to be synthetically lethal with the PARPinhibitor DPQ and were used as the positive control. The data shown hereis the average of six individual cell proliferation experiments wherecell survival was determined by measuring fluorescence after addition of10% v/v of Resazurin and incubation at 37° C. for 50 mins. Error barswere generated using the standard error of the mean;

FIG. 9 is a graph depicting synthetic lethality of PNKP and PARP.A549-VO=A549 vector only control cells and A549δPNKP=A549 cells in whichPNKP has been stably knocked-down. Values represented here are anaverage of no less than four independent cell proliferation experimentswhere cell survival was determined as described in FIG. 8. Error barswere calculated using standard error of the mean. There is an inverserelationship between the concentration of the PARP inhibitor3,4-dihydro-5[4-(1-piperindinyl)butoxy]-1(2H)-isoquinoline (DPQ) andcell survival of cells depleted of PNKP, however, there is no suchrelationship when comparing to control cells. This indicates that theco-disruption of PARP and PNKP causes synthetic lethality.

FIG. 10 is a graph depicting the lack of synthetic lethality betweenPNKP and DNA-PK. This graph shows that when M059J cells (DNA-PKcsnegative) are subjected to increasing concentrations of A12B4C3, nosynthetic lethality is seen. This data shows that co-disruption of PNKPand DNA-PK does not cause synthetic lethality. Values represented hereare an average of no less than four independent cell proliferationexperiments where cell survival was determined as described in FIG. 8;

FIG. 11 is a graph depicting synthetic lethality of PARP and DNA-PK anddemonstrates that when M059J (DNA-PKcs negative) and M059K (DNA-PKcspositive) cells are subjected to PARP inhibition using DPQ there is anapparent synthetically lethal relationship between DNA-PK and PARP (˜3:1ratio of survival between M059K and M059J cells when subjected to PARPinhibition). Values represented here are an average of no less than fourindependent cell proliferation experiments where cell survival wasdetermined as described in FIG. 8.

DETAILED DESCRIPTION

As will be described in more detail below, the present invention relatesto compounds, compositions, methods and kits for increasing thesensitivity of cells and/or tumours to chemotherapeutic agents and/orionizing radiation.

Also as will be discussed in more detail below, the present inventionrelates to inhibitors of polynucleotide kinase/phosphatase andpoly(ADP-ribose) polymerase.

In one aspect of the present invention, the compound(s) andcomposition(s) of the present invention inhibit PNKP phosphataseactivity. In specific examples of the present invention, the compound(s)and composition(s) of the present inhibit the DNA phosphatase activityof human PNKP or mouse PNKP.

As used herein, the term “inhibit” with respect to phosphatase activityis intended to include partial or complete inhibition of phosphataseactivity.

In accordance with one aspect of the present invention, there isprovided radiosensitizer and chemosensitizer compounds and compositions,methods and kits and the uses thereof.

The term “radiosensitizer”, as used herein refers to an agent, molecule,compound or composition that enhances the sensitivity of a neoplasticcell, a cancer cell and/or a tumor to the effects of radiation. The“sensitivity” of a neoplastic cell, a cancer cell, and/or a tumour toradiation is the susceptibility of the neoplastic cell, cancer cell,and/or tumour to the inhibitory effects of radiation on the cell's ortumour's growth and/or viability

The term “chemosensitizer”, as used herein, refers to an agent,molecule, compound or composition that enhances the sensitivity of aneoplastic cell, a cancer cell and/or a tumor to the effects of achemotherapeutic agent. The “sensitivity” of a neoplastic cell, a cancercell, and/or a tumour to a chemotherapeutic agent is the susceptibilityof the neoplastic cell, cancer cell, and/or tumour to the inhibitoryeffects of a chemotherapeutic agent on the cell's or tumour's growthand/or viability.

Compound(s)/Composition(s)

In one aspect of the present invention, the compound(s) of the presentinvention increase radiosensitivity and/or chemosensitivity of a cell(s)and/or tumour(s).

In another aspect of the present invention, the compound(s) of thepresent invention reduce cell survival of cells depleted with DNApolymerase β or PARP.

In another aspect of the present invention, the compound(s) of thepresent invention inhibits the phosphatase activity of PNKP.

The compounds of the present invention are capable of forming a varietyof different salts with various inorganic and organic acids. Such saltsare pharmaceutically acceptable for administration to a subject.

In a specific example, the compound(s) and composition(s) of the presentinvention increase sensitivity of a cell and/or tumour to radiation.

About half of patients with cancer are treated with radiation therapy,either alone or in combination with other types of cancer treatment.Radiation therapy (also referred to as radiotherapy, X-ray therapy, orirradiation) may be external, internal and systemic.

External radiation is delivered from a machine outside the body;internal radiation is implanted into or near the tumour(s); systemicradiation utilizes unsealed radiation sources.

External radiation therapy is used to treat most types of cancer,including but not limited to, cancer of the bladder, brain, breast,cervix, larynx, lung, prostate, and vagina. Intraoperative radiationtherapy (IORT) is a form of external radiation that is given duringsurgery, and can be used to treat localized cancers that cannot becompletely removed or that have a high risk of recurring in nearbytissues, including, but not limited to treatment of thyroid andcolorectal cancers, gynecological cancers, cancer of the smallintestine, and cancer of the pancreas. Prophylactic cranial irradiation(PCI) is another type of external radiation given to the brain when theprimary cancer (for example, small cell lung cancer) has a high risk ofspreading to the brain.

Internal radiation therapy (or brachytherapy) typically uses radiationsource sealed in a small holder called an implant. Implants may be inthe form of thin wires, plastic tubes called catheters, ribbons,capsules, or seeds. Interstitial radiation therapy, a type of internalradiation therapy) is inserted into tissue at or near the tumour site.It is used to treat tumors of the head and neck, prostate, cervix,ovary, breast, and perianal and pelvic regions. Intracavitary orintraluminal radiation therapy is inserted into the body with anapplicator. It is commonly used in the treatment of uterine cancer, andmay have application in other cancers, including breast, bronchial,cervical, gallbladder, oral, rectal, tracheal, uterine, and vaginal.

Systemic radiation therapy uses materials such as iodine 131 andstrontium 89, and may be taken by mouth or injected. Such therapy may beused in the treatment of cancers of the thyroid and adult non-Hodgkinlymphoma.

In a specific example, the radiation is γ-radiation. In one example, theionizing radiation is X-rays generated by a linear accelerator (Linac

In another example, the compound(s) and composition(s) of the presentinvention increases sensitivity of a cell(s) and/or tumour(s) to achemotherapeutic agent. In one example, the chemotherapeutic agent is atopoisomerase I inhibitor.

The term “topoisomerase I inhibitor” as used herein includes, but is notlimited to topotecan (HYCAMTIN®), gimatecan, irinotecan (CAMPTOSAR®),camptothecin and its analogues.

Indications, routes and methods of administration, and the like, oftopoisomerase I inhibitors are known to the skilled worker.

CAMPTOSAR® injection, for example, is indicated as a component offirst-line therapy in combination with 5-fluorouracil and leucovorin forpatients with metastatic carcinoma of the colon or rectum. CAMPTOSAR isalso indicated for patients with metastatic carcinoma of the colon orrectum whose disease has recurred or progressed following initialfluorouracil-based therapy.

HYCAMTIN for Injection, for example, is indicated for: metastaticcarcinoma of the ovary after failure of initial or subsequentchemotherapy, small cell lung cancer sensitive disease after failure offirst-line chemotherapy, combination therapy with cisplatin for stageIV-B, recurrent, or persistent carcinoma of the cervix which is notamenable to curative treatment with surgery and/or radiation therapy.

HYCAMTIN capsules, for example, is indicated for treatment of patientswith relapsed small cell lung cancer

In a specific example, the topoisomerase I inhibitor is camptothecin.

In accordance with a specific example of the present invention, thecompound(s) of the present invention increases sensitivity to ionizingradiation and/or chemotherapy, the compound(s) comprising:

2-(1-hydroxyundecyl)-1-(4-nitrophenylamino)-6-phenyl-6,7a-dihydro-1H-pyrrolo[3,4-b]pyridine-5,7(2H,4aH)-dione(A12B4C3),

2-(hydroxy(phenyl)methyl)-1-(4-nitrophenylamino)-6-phenyl-6,7a-dihydro-1H-pyrrolo[3,4-b]pyridine-5,7(2H,4aH)-dione(A1B4C3),

2-(hydroxy(3,4,5-trimethoxyphenyl)methyl)-1-(4-nitrophenylamino)-6-phenyl-6,7a-dihydro-1H-pyrrolo[3,4-b]pyridine-5,7(2H,4aH)-dione(A6B4C3),

tert-butyl2-(1-hydroxy-2,2-diphenylethyl)-6-methyl-5,7-dioxo-2,4a,5,6,7,7a-hexahydro-1H-pyrrolo[3,4-b]pyridin-1-ylcarbamate(A26B11C2), or

2-(hydroxy(thiophen-2-yl)methyl)-6-methyl-1-(phenylamino)-6,7a-dihydro-1H-pyrrolo[3,4-b]pyridine-5,7(2H,4aH)-dione(A39B1C2).

In another aspect of the present invention, there are providedpharmaceutical compositions and methods of treatment using suchpharmaceutical compositions for therapeutic uses.

In one example of the present invention, there is providedpharmaceutical compositions comprising A12B4C3, A1B4C3, A6B4C3, A26B11C2or A39B1C2 together with pharmaceutically acceptable diluents orcarriers.

Suitable pharmaceutical carriers include inert diluents or fillers,water and various organic solvents.

The pharmaceutical composition may, if desired, contain additionalingredients such as flavorings, binders, excipients and the like.

The compounds of the present invention are synthesized as has beendescribed before (12), the entire contents of this disclosure areincorporated herein by reference.

In another aspect of the present application, inhibitors of PNKP areused to reduce survival of cells depleted in DNA polymerase β. In oneexample, the inhibitor of PNKP is A12B4C3 (also referred to as H5herein, and in the Figures). In this example, A12B4C3 (also referred toas H5 herein, and in the Figures) reduced survival of DNA Polymerase βDominant Negative (Pol β DN) cells.

Accordingly, there is provided a potential synthetic lethal therapeuticstrategy for the treatment of cancers with specific DNA-repair defects,including those arising in carriers of a DNA Polymerase β mutation.]

In another aspect of the present application, inhibitors of PARP areused to reduce survival of cells depleted in PNKP or DNA-PK. In oneexample, the inhibitor of PARP is3,4-dihydro-5[4-(1-piperindinyl)butoxy]-1(2H)-isoquinoline (DPQ).

In one example, DPQ reduced survival of A549δPNKP (polynucleotidekinase/phosphatase (PNKP) depleted) cells. In one example, DPQ reducedsurvival of M059J (non-functional DNA-dependent protein kinase (DNA-PK))cells.

PARP inhibitors may be useful in the treatment of pancreatic cancer,solid tumours, melanoma, colorectal cancer, breast cancer, ovariancancer, non-small cell lung cell cancer, sarcoma, glioblastomamultiforme.

Additional examples of PARP inhibitors include, but are not limitd to,BSI401 (BiPar Science Inc.); CPH101 with CPH102 (Crimson Pharma);GPI21016 (Eisai Co.); ABT888 and ABT888 with Temozolomide (AbbottLaboratories); AZD2281 and AZD2281 with Avastin, Caelyx, Carboplatin,Carboplatin/paclitaxel, Dacadbazine, gemcitabine or paclitaxel(AstraZeneca Plc); MK4827 (Merk & Co. Inc); AZD2281 and AZD2281 withcisplatin or paclitaxel (AstraZeneca Plc); BSI201 and BSI201 withcarboplatin/paclitaxel, chemotherapy, irinotecan, temodar and radiation;or topotecan (BiPar Science Inc); AG014699 or AG14699 with temozolomide(Pfizer Inc); BSI201 with gencitabine and carboplatin; PARP 1 Sentineal(Sentinel oncology).

The following Table depicts examples non-limiting example PARPinhibitors, the phase of testing and indication(s).

Company Product Phase Indication BiPar Sciences, BSI401 PC PancreaticCancer Inc. Crimson Pharma CPH101 with PC Cancer CPH102 Eisai Co.GPI21016 PC Cancer (Cancer Chemosensiti- zation and Radio-sensitization) Abbott ABT888 I Cancer Laboratories ABT888 with I SolidTumors Temozolomide AstraZeneca AZD2281 with I Solid Tumors Plc Avastin(Advanced Solid Tumors) AZD2281 with I Solid Tumors Caelyx (AdvancedSolid Tumors) AZD2281 with I Solid Tumors Carboplatin (Advanced SolidTumors) AZD2281 with I Solid Tumors Carboplatin, (Advanced PaclitaxelSolid Tumors) AZD2281 with I Melanoma Dacarbazine (Advanced Melanoma)AZD2281 with I Pancreatic Cancer Gemcitabine AZD2281 with I Solid TumorsPaclitaxel (Advanced Solid Tumors) BiPar Sciences, BSI201 I Solid TumorsInc. (Solid Tumors (Monotherapy)) BSI201 I Solid Tumors Cephalon IncCEP9722 I Solid Tumors Merck & Co Inc MK4827 I Solid Tumors (OvarianNeoplasm) AstraZeneca Plc AZD2281 II Colorectal Cancer AZD2281 II BreastCancer (Advanced Breast Cancer) AZD2281 II Ovarian Cancer (BRCADeficient Advanced Ovarian Cancer) AZD2281 with II Breast CancerCisplatin (Triple Negative Breast Cancer) AZD2281 with II Breast CancerPaclitaxel (Metastatic Triple Negative Breast Cancer) BiPar Sciences,BSI201 II Pancreatic Cancer Inc. (BRCA-Negative Pancreatic Cancer)BSI201 II Ovarian Cancer (BRCA-Negative Ovarian Cancer (Monotherapy))BSI201 with II Cancer (Uterine Carboplatin, Carcinosarcoma) PaclitaxelBSI201 with II Non-Small-Cell Carboplatin, Lung Cancer Paclitaxel BSI201with Chemo- II Sarcoma therapy BSI201 with II Breast Cancer Irinotecan(Metastatic Breast Cancer) BSI201 with II Brain Tumor (Newly Temodar andDiagnosed Radiation Therapy Glioblastoma Multiforme) BSI201 with IIOvarian Cancer Topotecan (Advanced Ovarian Cancer) Pfizer Inc (PFE)AG014699 II Breast Cancer AG14699 II Cancer AG14699 II Ovarian CancerAG14699 with II Melanoma Temozolomide (Metastatic Malignant Melanoma)BiPar Sciences, BSI201 with III Breast Cancer Inc. (Private) Gemcitabine(Metastatic Triple and Carboplatin Negative Breast Cancer) LEAD PARPInhibitor NA Cancer Therapeutics, Program Inc. (Private) LEAD THERAPEU-TICS Sentinel PARP 1 SENTINEL NA Solid Tumors Oncology (Tumors)

Thus, in one aspect of the present invention there is provided apotential synthetic lethal therapeutic strategy for the treatment ofcancers with specific DNA-repair defects, including those arising incarriers of a PNKP or DNA-PK mutation.

Compounds of the present invention may be administered with aphysiologically acceptable carrier. A physiologically acceptable carrieris a formulation to which the compound can be added to dissolve it orotherwise facilitate its administration. Non limiting examples include,but are not limited to, water, saline, physiologically buffered saline.

Solid dosage forms for oral administration can include capsules,tablets, pills, powders, and granules. In such forms, the compounds ofthe present invention may be combined with one or more adjuvants, asindicated by the route of administration. Compounds of the presentinvention can be admixed with, for example, lactose, sucrose, starchpowder, cellulose esters of alkanoic acids, cellulose alkyl esters,talc, stearic acid, magnesium stearate, magnesium oxide, sodium andcalcium salts of phosphoric and sulfuric acids, gelatin, acacia gum,sodium alginate, polyvinylpyrrolidone, and/or polyvinyl alcohol, andthen tableted or encapsulated for convenient administration. Suchcapsules or tablets can contain a controlled-release formulation as canbe provided in a dispersion of active compound in hydroxypropylmethylcellulose. In the case of capsules, tablets, and pills, the dosage formscan also comprise buffering agents such as sodium citrate, magnesium orcalcium carbonate or bicarbonate. Tablets and pills can additionally beprepared with enteric coatings.

Compounds and pharmaceutically acceptable compositions of the presentinvention can be administered by parenteral administration, in the formof aqueous or non-aqueous isotonic sterile injection solutions orsuspensions. These solutions and suspensions can be prepared fromsterile powders or granules having one or more of the carriers ordiluents mentioned for use in the formulations for oral administration.Compounds of the present invention can be dissolved in water,polyethylene glycol, propylene glycol, ethanol, corn oil, cottonseedoil, peanut oil, sesame oil, benzyl alcohol, sodium chloride, and/orvarious buffers. Other adjuvants and modes of administration are welland widely known in the pharmaceutical art, as know by the skilledworker. P Liquid dosage forms for oral administration includepharmaceutically acceptable emulsions, solutions, suspensions, syrups,and elixirs containing inert diluents commonly used in the art, such aswater. Such compositions can also comprise adjuvants, such as wettingagents, emulsifying and suspending agents, and sweetening, flavoring,and perfuming agents.

It will be appreciated that the amount of active ingredient that can becombined with the carrier materials to produce a single dosage formvaries depending upon the mammalian host treated and the particular modeof administration.

The compounds and compositions of the present invention are suitable forcombination. Combination therapy as used herein includes administrationof the therapeutic agents in a sequential manner, wherein eachtherapeutic agent is administered at a different time, as well asadministration of the therapeutic agents at the same time.

As used herein, the therapeutic agents are administered in a sequentialmanner, wherein each therapeutic agent is administered at a differenttime, or administered in a generally simultaneous manner. The generallysimultaneous administration can be accomplished, for example, byadministering to the subject a single capsule having a fixed ratio ofeach therapeutic agent or in multiple, single capsules for each of thetherapeutic agents.

Administration of each therapeutic agent, whether sequential orgenerally simultaneous, can be effected by any appropriate routeincluding, but not limited to, oral routes, intravenous routes,intramuscular routes, and direct absorption through mucous membranetissues, etc. The therapeutic agents can be administered by the sameroute or by different routes.

Combination therapy also includes administration of the therapeuticagents in combination with other biologically active ingredients (suchas, but not limited to, a second and different antineoplastic agent) andnon-drug therapies (such as, but not limited to, surgery or radiationtherapy).

Where the combination therapy further comprises radiation therapy, theradiation therapy may be conducted at a suitable time so long as abeneficial effect from the co-action of the combination of thetherapeutic agents and radiation treatment is achieved.

Method(s)

In accordance with another aspect of the present invention, there isprovided a method(s) for increasing the sensitivity of a cell(s) and/ortumour(s) to chemotherapeutic agents and/or ionizing radiation.

In accordance with another aspect of the present invention, there isprovided a method(s) for inhibiting PNKP phosphatase activity. In oneaspect of the present invention, the PNKP is human PNKP. In one aspectof the present invention, the PNKP is mouse PNKP. In another example,the PNKP is a mammalian PNKP.

In one aspect of the present invention, increasing the sensitivity of acell(s) and/or tumour(s) to chemotherapeutic agents and/or ionizingradiation is carried out in vitro, including, but not limited to, incultured cells. In a specific example, the cultured cells are A549and/or MDA-MB-231 cells.

In one aspect of the present invention, inhibition of the phosphataseactivity of PNKP is carried out in vitro, including, but not limited to,in cultured cells. In a specific example, the cultured cells are A549and/or MDA-MB-231 cells.

In another aspect of the present invention, increasing the sensitivityof a cell(s) and/or tumour(s) to chemotherapeutic agents and/or ionizingradiation, and inhibiting the phosphatase activity of PNKP, is carriedout in vivo in a subject

The term “subject”, as used herein, refers to any human or animal whomwould benefit from treatment with a chemosensitizer and/or aradiosensitizer, and/or has a disorder associated with PNKP.Non-limiting examples of a subject include humans, non-human mammal,rodents, companion animals, livestock and the like.

The compound(s) and composition(s) according to the present inventionare chemosensitizers and/or radiosensitizers useful for the treatment ofcancer. In one aspect of the present invention, the methods, compound(s)and composition(s) of the present invention may be used for thetreatment of neoplasia disorders including benign, metastatic andmalignant neoplasias.

Another embodiment of the present invention relates to treating orlessening the severity of one or more diseases in which PNKP is known toplay a role. In one example, the disease is cancer.

By the terms “treating” or “lessening the severity”, it is to beunderstood that any reduction using the methods, compounds andcomposition disclosed herein, is to be considered encompassed by theinvention. Treating or lessening in severity, may, in one embodimentcomprise enhancement of survival, or in another embodiment, haltingdisease progression, or in another embodiment, delay in diseaseprogression, or in another embodiment, diminishment of pain, or inanother embodiment, delay in disease spread to alternate sites, organsor systems. Treating or lessening in severity, may, in one embodiment,comprise a reduction in the amount/dosage of radiotherapy and/orchemotherapy otherwise required to treat a subject, thereby resulting ina reduction of normal tissue damage. It is to be understood that anyclinically beneficial effect that arises from the methods, compounds andcompositions disclosed herein, is to be considered to be encompassed bythe invention.

The term “pharmaceutically effective amount” as used herein refers tothe amount of a drug or pharmaceutical agent that will elicit thebiological or medical response of a tissue, system, animal or human thatis being sought by a researcher or clinician. This amount can be atherapeutically effective amount.

In accordance with another aspect of the present invention, there isprovided a method(s) for the treatment of a subject, including a human,suffering from a cancer comprising administering to said subject acompound comprising A12B4C3, A1B4C3, A6B4C3, A26B11C2 or A39B1C2, apharmaceutically acceptable salt, in combination with chemotherapyand/or radiotherapy.

In accordance with another aspect of the present invention, there isprovided a method for the treatment of a subject suffering from adisorder, such as cancer, associated with a defect in DNA polymerase β,comprising administering to said subject an inhibitor of PNKP. In oneexample the inhibitor of PHNP is A12B4C3 (also referred to as H5 herein,and in the Figures).

Studies have suggested that ˜30% of human tumours express DNA Polymeraseβ variants (see Starcevic D et al. (2004) Cell Cycle 3: 998-1001). Thehighest proportions of variants are in gastric, colorectal andesophageal cancers, and also been found in lung, breast, prostate andbladder cancers.

In accordance with another aspect of the present invention, there isprovided a method for the treatment of a subject suffering from adisorder, such as cancer, associated with a defect in PNKP or DNA-PK,comprising administering to said subject an inhibitor of PARP. In oneexample the inhibitor of PARP is DPQ.

As used herein, the term “pharmaceutically acceptable salt” refers tothose salts which are, within the scope of sound medical judgment,suitable for use in contact with the cell(s) and/or tissue(s) of asubject without undue toxicity, irritation, allergic response and thelike, and are commensurate with a reasonable benefit/risk ratio.

Methods of the present invention are conveniently practiced by providingthe compound(s) and/or composition(s) used in such method in the form ofa kit. Such a kit preferably contains the instructions of the usethereof.

To gain a better understanding of the invention described herein, thefollowing examples are set forth. It should be understood that theseexample are for illustrative purposes only. Therefore, they should notlimit the scope of this invention in any way.

EXAMPLE-I

Materials and Methods

Enzymes

Recombinant human PNKP was purified as described previously (14, 29) andstored in 50 mM Tris-HCl, pH 7.4, 100 mM NaCl, 5 mM MgCl₂, 0.5 mMdithiothreitol. Recombinant mouse PNKP was purified as previouslydescribed (25). Schizosaccharomyces pombe PNKP was purified as describedpreviously (30). Phage T4 polynucleotide kinase (T4 PNK) was purchasedfrom Roche Diagnostics (Indianapolis, Ind.). Human PP-1cγ protein wasexpressed in E. coli and purified as previously described (31). Ratδ-calcineurin protein was expressed in E. coli and purified aspreviously described (32).

Human recombinant aprataxin (APTX) protein with an N-terminal 6× His tagwas expressed in BL21-Gold (DE3) E. coli competent cells (Stratagene, LaJolla, Calif.) using the QIAgene expression construct (Qiagen,Mississauga, ON). A single colony of kanamycin resistant E. coli wasused to inoculate a 200-ml overnight culture (e.g, about 12 to about 16hours in Luria-Bertani (LB) media containing 30 μg/ml kanamycin. Four50-ml fractions of overnight culture were then subcultured into 4×1 L LBwithout kanamycin. Once the culture reached an optical density of ˜0.6at 600 nm, protein expression was induced using 0.2 mMisopropyl-1-thio-β-galactopyranoside (Sigma, St Louis, Mo.) at 37° C.for 2 hours. Cells were harvested by centrifugation at 10,000 rpm for 10minutes at 4° C. and resuspended in 40 ml buffer (50 mM NaH₂PO₄, 250 mMNaCl, 1 mM PMSF, at pH 7.9). The solution was then stirred on ice for 30minutes in the presence of 30 mg lysozyme and 4 mg PMSF, 1 μg/ml each ofpepstatin and leupeptin. The bacteria were then sonicated 6×30 secondsallowing 30 seconds between intervals to cool down. The cell debris wasthen spun down at 15,000 rpm for 15 minutes at 4° C. and the supernatantcollected. The supernatant was then stirred on ice in the presence of 4ml Probond resin (Invitrogen, Burlinton, ON) for 1 hour and then loadedonto a column. The resin was washed with 3×5 ml 20 mM imidiazole and 5ml fractions were collected. Then, 25 ml of 150 mM imidazole was loadedonto the column and 1 ml fractions were collected. Fractions were run ona 10% SDS-PAGE and stained with Coomassie Brilliant Blue R-250(Invitrogen). Fractions showing high concentrations and single bandswere then combined and concentrated using a 30 kDa cutoff AmiconUltra-15 centrifugal filter (Millipore, Etibicoke, ON) and dialyzed with50 mM Tris-HCl (pH 7.5), 100 mM NaCl, and 5 mM MgCl₂. His-APTXconcentration was then determined using the Bio-Rad Protein assay(Bio-Rad, Mississauga, ON).

Cells

A549 (human lung carcinoma cells) and MDA-MB-231 (human breastadenocarcinoma cells) were obtained from the American Type CultureCollection (Manassas, Va.). Cells were cultured in a 1:1 mixture ofDulbecco's modified Eagle's medium/nutrient mixture F-12 (DMEM/F-12)supplemented with 10% fetal calf serum (FCS), penicillin (50 U/ml),streptomycin (50 μg/ml), L-glutamine (2 mM), non-essential amino acids(0.1 mM) and sodium pyruvate (1 mM), and maintained at 37° C. under 5%CO₂ in a humidified incubator. All culture supplies were purchased fromInvitrogen. The generation of PNKP-depleted A549 cells, termed“A549δNKP” (also referred to as C-ter3), has been previously described(24). The PNKP-depleted MDA-MB-231, termed “MDA-MB-231δPNKP”, cells weregenerated in a similar fashion except that the shRNA-expressing pSUPERvector used on this occasion (pSUPER.neo, OligoEngine, Seattle, Wash.)also contained the cDNA for the G418 selectable marker.

Optimization of Fluorescence Quenching-Based Assay for PNKP3′-Phosphatase Activity.

The Lightspeed™ assay developed originally for protein kinases by QTLBiosystems (Santa Fe, N. Mex.) was modified. The standard substrate usedfor this assay was a 20-mer oligonucleotide (5′-TAMRA-AAT ACG AAT GCCCAC ACC GC-P-3′) (SEQ ID NO: 1) labelled with5′-(6-carboxytetramethylrhodamine) at the 5′-end and bearing a terminal3′-phosphate (Integrated DNA Technologies, Coralville, Iowa). TheTAMRA-labelled oligonucleotide lacking a 3′-phosphate served as acontrol. Four standard solutions, consisting of 0, 25, 50 and 100%3′-phosphorylated oligonucleotide, were prepared by mixing the twooligonucleotides in respective proportions (2.5 nM total oligonucleotideconcentration). The assay was performed in 384-well white Optiplatemicroplates (PerkinElmer, Woodbridge, ON) in 70 mM Tris-HCl, pH 7.4, 60mM MgCl₂, 5 mM MnCl₂, 0.3% BSA, 0.09% sodium azide. Reaction buffer wasprepared by adding 1 mM DTT immediately prior to use. Five μL of3′-phosphatase substrate (final concentration 0.5 μM) was used per well.In duplicate, 10 μl of each concentration of hPNKP was added per well.Plates were incubated for 1 hour at 37° C. and then 15 μl of 1× sensorsolution (provided by QTL) was added to each well and incubated for 30minutes at room temperature. Fluorescence (485 nm excitation and 520 nmemission wavelengths) of each well was read in a FLUOstar Optima® (BMGLabtech Inc, Durham, N.C.). Data were analyzed using GraphPad Prism®Software (San Diego, Calif.).

Screening the Small Molecule Library

A library of 244 small molecules (28) was used for the screening. Smallmolecules were provided in powder form and were dissolved in 100% DMSOand a final concentration of 100 μM was added to each well and assayswere performed as described above.

After obtaining an optimum calibration curve and enzyme concentrationcurve, a simplified form of the assay to test the library in a shorttime, was employed. One concentration of hPNKP, 50 ng, was tested andcompared with the control well with no enzyme. The assay was conductedin the same way as described above with respect to oligonucleotides,buffer, controls, incubation lengths/temperatures, centrifugations andsensor addition.

Assay for 3′-Phosphatase Activity Based on the Release of InorganicPhosphate (Pi).

hPNKP phosphatase reactions (20 μl total volume) were set up as follows:1 hPNKP (100 ng), 2 μl 10× phosphatase buffer (500 mM Tris-HCl, pH 7.4,0.1 mM EDTA, 1 mM spermidine and 2.5 mM DTT), 2 μl of 1 mM 3′-P 20 meroligonucleotide, 15 μl distilled H₂O and 1 μl small molecule (varyingconcentrations). (The oligonucleotide had the same sequence as that usedin the fluorescence quenching assay, but without the TAMRA substituent).The reactions were then transferred to a clear polystyrene colorimetric384-well plate and incubated at 37° C. for 30 minutes. PiColorlock Goldreagent (Innova Biosciences Ltd., Cambridge, UK) was prepared shortlybefore use by addition of 1/100 vol. of accelerator to PiColorlock Goldreagent as directed by the manufacturer. The Gold mix was then added toPi-containing samples in a volume ratio of 1:4 and the samples were thenincubated at room temperature for 30 minutes before the absorbance wasread at 620 nm using a FLUOstar Optima® plate reader (BMG Labtech Inc.Durham, N.C.)

Conventional Radio-Gel Assay for hPNKP 3′-Phosphatase Activity

hPNKP phosphatase activity was determined by monitoring the removal ofthe 3′-phosphate from a 5′-³²P-labeled 20 mer oligonucleotide containinga 3′-phosphate (5′-ATT ACG AAT GCC CAC ACC GC-P-3′) (SEQ ID NO: 2) aspreviously described (14). Briefly, 5′-end of the oligomer was labelledby incubation with phage T4 phosphatase-free polynucleotide kinase(Roche Diagnostics, Indianapolis, Ind.) and [γ-³²P]ATP (PerkinElmer).The labelled oligomer was then incubated with hPNKP for 20 minutes andthe level of 3′-dephosphorylation was monitored by electrophoresis on a12% polyacrylamide/7 M urea sequencing gel for 3 hours in 1× TBE buffer.Gels were scanned with a Typhoon 9400 Variable Mode Imager (GEHealthcare, Little Chalfont, UK), and quantified using Image Quant 5.2Software (GE Healthcare).

PP-1cγ and Calcineurin Phosphatase Assay

PP-1cγ and calcineurin activity was analyzed, using a colorimetricp-nitrophenol phosphate (pNPP) assay as previously described (33). Thereaction were carried out in a 96-well microplate with a final volume of60 μl containing 40 μl of pNPP assay buffer (50 mM Tris, pH 7.4, 0.1 mMEDTA, 30 mM MgCl₂, 0.5 mM MnCl₂, 1 mg/ml BSA, 0.2% β-mercaptoethanol),0.03 μg PP-1cγ (specific activity >30 units per mg) or a catalyticallyequivalent quantity of calcineurin and 10 μl of 0.5 mM DNA3′-phosphatase inhibitor sample in DMSO or 10 μl of 50 μM A12B4C3 samplein DMSO, or control solvent. After a 10-minute incubation at 37° C., 10μl of 30 mM pNPP was added to each well and incubated for an additionalof 60 minutes and 45 minutes for PP-1cγ and calcineurin, respectively.The absorbance at 405 nm was measured using a SOFTmax 2.35 microplatereader (Molecular Devices, Sunnyvale, Calif.).

DNA Kinase Assay

Reaction mixtures (20 μl) containing kinase buffer (80 mM succinic acid(pH 5.5), 10 mM MgCl₂, and 1 mM DTT), 100 μM 20-mer oligonucleotidesubstrate, 3.3 pmol of [γ-32P]ATP, A12B4C3 (0-50 μM) in 2 μl DMSO, and 1μg of PNK were incubated at 37° C. for 20 minutes. The reaction wasstopped by addition of an equal volume of DNA loading dye (90%formamide, 0.02% bromophenol blue, 0.02% xylene cyanol in 1× TBE).Samples were boiled for 5 minutes and the products separated on a 12%polyacrylamide/8 M urea gel. Gels were scanned with a Typhoon 9400Variable Mode Imager (GE Healthcare), and quantified using Image Quant5.2 Software (GE Healthcare).

Cell Proliferation Assay

To determine the effect of the inhibition of PNKP by small moleculeinhibitors on cell proliferation we used the CellTiter 96™ AQueousNon-Radioactive Cell Proliferation Assay (Promega, Madison Wis.), betterknown as the MTS assay. Approximately 2.5×10³ A549 cells were plated intriplicate in a 96-well plate with different concentration of A12B4C3.After 72 hours, 20 μl of CellTiter 96 Aqueous One Solution Reagent wasadded to each well and cells were incubated for 4 more hours at 37° C.The absorbance recorded at 490 nm was used as the number of living cellsin culture (FLUOstar Optima, BMG Labtechnologies).

Cytotoxicity Studies

The effect of hPNKP inhibition by A12B4C3 on cellular survival followingexposure to ionizing radiation was measured in A549, A549δPNKP andMDA-MB-231 and MDA-MB-231δPNKP cells by clonogenic assays. Cells wereseeded on 60-mm tissue culture plates at various concentrations to givebetween about 100-1000 colonies per plate and returned to the incubatorovernight to allow the cells to attach. For radiosensitization studies,the cells were incubated with or without 1 μM A12B4C3 for 2 hours beforeirradiation and then exposed to increasing doses of γ-radiation (⁶⁰CoGammacell; Atomic Energy of Canada Limited, Ottawa). After irradiation,cells were incubated for a further 24 hours in the same media and thenwashed twice with phosphate-buffered saline (PBS) and incubated in freshmedia without the inhibitor. Colonies were stained with crystal violetafter 10 to 14 days and counted with an automated Colcount colonycounter (Oxford Optronix, Oxford, UK).

The effect of hPNKP inhibition by A12B4C3 on cellular survival followingexposure to the topoisomerase I poison, camptothecin, was measured inA549 and A549δPNKP cells by the clonogenic survival assay (colonyforming assay). Cells were seeded on 60-mm tissue culture plates atvarious concentrations to give between about 100-1000 colonies per plateand returned to the incubator overnight to allow the cells to attach.For chemosensitization studies, the cells were incubated with or without1 mM A12B4C3 for 2 hours before addition of camptothecin and thenexposed to increasing doses of camptothecin (Sigma). After addition ofthe topoisomerase I poison, cells were incubated for a further 24 hoursin the same media and then washed twice with phosphate-buffered saline(PBS) and incubated in fresh media without the inhibitor. Colonies werestained with crystal violet after 10 to 14 days and counted with anautomated Colcount colony counter (Oxford Optronix, Oxford, UK).

Results

Screening of the Library by a Fluorescence-Based Assay

The fluorescence-based phosphatase assay adapted was originallydeveloped to monitor protein phosphatase activity (34). This assayinvolves a fluorescent sensor molecule coated in trivalent metal cationswhich causes superquenching of the sensor signal when in close proximityto a dye (TAMRA) on the substrate. The sensor is brought close to TAMRAvia the ionic bond formed between the terminal DNA phosphate and thetrivalent metal cations on the sensor. Removal of the phosphate leads toan elevation of fluorescence because the sensor is not brought closeenough to TAMRA for its signal to be quenched. The buffer conditionswere modified so that the internucleotide phosphate groups of a DNAsubstrate would not interfere strongly with the process of measuring thepresence of a terminal phosphate group as shown by the standard curve of0, 25, 50 and 100% phosphorylated oligo solutions (FIG. 1A). The amountof hPNKP required for near complete dephosphorylation of theoligonucleotide was determined by measuring the fluorescence signal as afunction of hPNKP present in the reaction (FIG. 1B), and as a result 50ng was chosen as the standard quantity of hPNKP for each reaction in thescreen. Heat inactivated hPNKP was used as a control.

A chemical library containing over 200 polysubstituted piperidenemolecules (28) was screened for their capacity to inhibit thephosphatase activity of human PNKP. Five of the compounds, A12B4C3,A1B4C3, A6B4C3, A26B11C2 and A39B1C2, were observed to cause significantinhibition as shown in FIG. 1C. Also shown are the data for three othercompounds, A4B8C2, A28B3C1 and A24B12C3, as examples of the majority ofcompounds that failed to inhibit hPNKP. The chemical names andstructures of these compounds are shown in FIG. 2.

Confirmation of Inhibition of PNKP Phosphatase Activity

A conventional radio-gel assay was used to verify the inhibition ofhPNKP phosphatase activity by these small molecules. This assay shows ashift on an acrylamide sequencing gel that corresponds to 3′-phosphateremoval from a 20-mer single-stranded oligonucleotide (35). Examples ofthe assay are shown in FIG. 3. All five of the positively identifiedcompounds, namely A12B4C3, A1B4C3, A6B4C3, A26B11C2 and A39B1C2,inhibited hPNKP phosphatase activity. A number of small molecules shownby the screening assay not to be inhibitors of hPNKP phosphataseactivity was also examined, and they also failed to show inhibition bythe radio-gel approach (data not shown)

Inhibitory Activity and Specificity of A12B4C3

To further assess the activity of the five inhibitory molecules aproprietary colorimetric reagent (PiColorLock Gold) was used thatmeasures release of inorganic phosphate. A drawback encountered with thefluorescence-based approach can be fluorescence quenching arising fromdirect interaction of the small molecule with the sensor agent. (Note inFIG. 1C the lower fluorescence signal of the sensor caused by exposureto some compounds in the absence of PNKP). This problem is avoided inthe colorimetric assay, which measures the release of inorganicphosphate (Pi) from a 3′-phosphorylated 20-mer oligonucleotide based onthe change in absorbance of malachite green in the presence ofmolybdate. Based on the standard curve obtained using 0, 25, 50, and100% phosphorylated substrates (FIG. 4A), it was found that A12B4C3 wasthe most potent of the five PNKP inhibitors (FIG. 4B) and obtained anIC₅₀ dose of 0.06 μM and near maximal inhibition with a concentration of5 μM (FIG. 4C). IC₅₀ values for the other compound, which also containsa nitrobenzylamine side chain, A6B4C3, was determined and found to besomewhat higher (˜0.3 μM).

Specificity of A12B4C3

To determine the specificity of A12B4C3 for human PNKP phosphataseactivity, a number of closely related phosphatases such as the PNKPenzymes isolated from bacteriophage T4, Schizosaccharomyces pombe andmouse, as well as aprataxin, were examined. It was observed that 50 μMA12B4C3 inhibited phage T4 and the S. pombe PNKPs by ˜12.5 and ˜20%,respectively, compared to ˜85% inhibition of human PNKP (FIG. 5A). Thecompound significantly inhibited mouse PNKP (which shares ˜80% identityto human PNKP) at all concentrations tested (down to 5 μM), though notquite as effectively as human PNKP (FIG. 5B).

It was also tested whether A12B4C3 could inhibit aprataxin, which isanother human DNA 3′-phosphatase. For this experiment, theoligonucleotide substrate was incubated with equal quantities of the twoenzymes that were purified from bacteria on the same day. It wasobserved that aprataxin has a robust phosphatase activity that wasrefractory to 50 μM A12B4C3 (FIG. 5C).

Two well known protein phosphatases, calcineurin and PP-1γ were alsoexamined. Neither enzyme displayed inhibition when treated with anA12B4C3 concentration as high as 83.3 μM, whereas the control inhibitormicrocystin LR reduced the activity of PP1 ˜65% (FIG. 5D).

The effect of A12B4C3 on the kinase activity of human PNKP was examinedby quantifying the transfer of ³²P-labeled phosphate from radiolabeledATP to an oligonucleotide. As shown in FIG. 5E, 50 μM A12B4C3 reducedhuman PNKP activity by ˜30% but at 1 μM the inhibition was reduced to˜16%. However, the inhibition of the kinase and phosphatase activitiescannot be directly compared because the standard assay for kinaseactivity uses ten-fold more enzyme than the assay for phosphataseactivity

Cytotoxicity of A12B4C3 and Cellular Radio-Sensitization andChemo-Sensitization by A12B4C3

The foregoing data indicated that A12B4C3 is a potent inhibitor of humanPNKP in vitro. The compound's effectiveness as a radiosensitizer wasexamined.

A12B4C3 was first tested for inherent toxicity. Cytotoxicity wasmeasured by cell proliferation assay after exposure of A549 human lungadenocarcinoma cells and MDA-MB-231 cells to different doses of thecompound for 72 hours (FIG. 6A). A dose-dependent reduction in cellproliferation up to 50% at 100 μM A12B4C3 was observed. No furtherdecrease was seen at higher doses. While not wishing to be bound bytheory, this may be indicative of a maximal level of drug uptake. Noeffect on cell proliferation was detected after exposure to 1 μMA12B4C3. The lack of cytotoxicity at this dose was confirmed byclonogenic survival assay following exposure to A12B4C3 up to 24 hours(data not shown).

The capacity of A12B4C3 to act as a radiosensitizer was then examined.A549 cells were incubated with 1 μM A12B4C3 for 2 hours prior toirradiation and then maintained in the presence of the compound for afurther 24 hours. The survival curves indicated that exposure to A12B4C3almost doubled the radiosensitivity of A549 cells (FIG. 6B). Thisradiation response was nearly identical to that seen with cells depletedof PNKP by stable expression of shRNA (A549δPNKP). On the other hand,A12B4C3 failed to sensitize the PNKP-depleted cells. Similar data wereobtained with wild type and PNKP-depleted MDA-MB-231 breast cancer cells(FIG. 6C).

The capacity of A12B4C3 to act as a chemosensitizer was then examined.A549 and A549δPNKP cells were seeded on 60-mm tissue culture plates atvarious concentrations to give between about 100-1000 colonies per plateand returned to the incubator overnight to allow the cells to attach.The cells were incubated with or without 1 mM A12B4C3 for 2 hours beforeaddition of camptothecin and then exposed to increasing doses ofcamptothecin (Sigma). After addition of camptothecin, cells wereincubated for a further 24 hours in the same media and then washed twicewith phosphate-buffered saline (PBS) and incubated in fresh mediawithout the inhibitor. Colonies were stained with crystal violet after10 to 14 days and counted with an automated Colcount colony counter(Oxford Optronix, Oxford, UK).

Discussion

The increased interest in therapeutics based on DNA repair inhibitionhas led to the discovery of several small molecule inhibitors of key DNArepair proteins including MGMT, PARP, ATM, DNA-PK, APE1, and Tdp1 (1,4-6). Reduction in the activity of each of these enzymes sensitizescells to a selection of chemotherapeutic agents and, in some cases,ionizing radiation. Previous studies suggested that PNKP depletion,mediated by shRNA, sensitizes cells to ionizing radiation, camptothecinand the alkylating agent, methyl methanesulphonate (24).

PNKP possesses 5′-kinase and 3′phosphatase activity. This necessitatedthe development of a suitable screening assay for inhibitors of thephosphatase activity. Most fluorescence-based high throughput screeningassays for phosphatase activity have been directed towards proteinphosphatases and rely on immunodetection using antibodies to thephosphorylated peptide substrate. The superquenching assay, originallydevised by Rininsland et al. (34), presented an alternative approachthat depended on the presence of a phosphate group for chemicalrecognition. It required some optimization involving protonation of thesubstrate to enhance the influence of the terminal phosphomonoestergroup over the internucleotide phosphodiester groups of the DNAsubstrate. Using this protocol a Z-factor of 0.68 was obtained, which isconsidered sufficient for identification of inhibitors in highthroughput screens. The inhibitory activity of compounds identified bythe superquenching assay was corroborated by the conventional radio-gelassay and by the PiColorlock colorimetric assay.

The chemical library of polysubstituted piperidines proved a relativelyrich source of inhibitory compounds. The three most active compoundscontain a nitrobenzylamine substituent on the ring nitrogen of the sixmembered ring of the piperidine (FIG. 2). The importance of thissubstituent to the binding of the inhibitor to PNKP remains to bedetermined. Of the three compounds, A12B4C3 was the most effectiveinhibitor of PNKP with an IC₅₀ of 0.5 μM compared to ˜1 μM for the othertwo compounds (FIG. 4C). In addition to the nitrobenzyl substituent,A12B4C3 also features a long hydrophobic alkyl chain.

An important issue with all small molecule inhibitors is theirspecificity. The response of a number of other phosphatases to A12B4C3was examined. Phage T4 polynucleotide kinase and human PNKP sharesimilar nucleic acid kinase and phosphatase activities. However, withthe exception of the enzyme active sites, the proteins bear norecognizable homology (25, 27). The phosphatase domains of both proteinsbelong to the haloacid dehalogenase (HAD) superfamily (25, 36, 37) witha conserved DxDGT motif, where the first Asp forms a covalentphospho-aspartate intermediate with the substrate. While not wishing tobe bound by theory, that A12B4C3 failed to inhibit T4 PNK (FIG. 5A)suggests that the small molecule does not directly interact with thisconserved HAD motif. The catalytic domain (phosphatase and kinase) of S.pombe PNKP, on the other hand, shares considerably more structuralsimilarity with human PNKP than the T4 enzyme, with 127 identicalresidues, including the HAD motif (30). Despite this level of sequenceoverlap, the inhibition of S. pombe PNKP by A12B4C3 was limited (˜20%),even at 50 μM inhibitor concentration (FIG. 5A). Again, while notwishing to be bound by theory, this may suggest that the compoundinteracts primarily with a region specific to mammalian PNKP, hence thestrong inhibition of human and mouse PNKP (FIG. 5B).

A12B4C3 displayed no inhibition (FIG. 5D) of either of the two proteinphosphatases tested, protein phosphatase 1 (PP-1) and calcineurin(protein phosphatase 2B), which are members of the eukaryoticserine/threonine family involved in a broad range of signal transductionpathways (38).

The possibility that A12B4C3 interacts with other protein phosphatasesor indeed other enzymes can not be ruled out, but the tests forradiosensitization by A12B4C3 (FIG. 6B and C) indicated not only thatthe compound effectively sensitized wild type cells to ionizingradiation, but also revealed that PNKP is most likely the cellulartarget for A12B4C3 in human cells because it failed to sensitize thePNKP-deficient cells.

The tests for chemosensitization by A12B4C3 (FIG. 7) indicated that thecompound effectively sensitized wild type cells to camptothecin.

EXAMPLE-II

Materials and Methods

Referring to FIGS. 8-11, to examine synthetic lethality, a3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium(MTS) fluorescence-based cell proliferation assay was used together withcell lines depleted of specific DNA repair proteins using shRNA, smallmolecule inhibitors of DNA repair proteins, or expression of dominantnegative fragments of repair proteins.

Typically, 2,500 A549, Clone 13 (polynucleotide kinase/phosphatase(PNKP) depleted), DNA Polymerase β Dominant Negative (Pol β DN), M059J(non-functional DNA-dependent protein kinase (DNA-PK)), or M059K(functional DNA-PK) cell lines were plated in a 96-well plate in 200 μLDMEM/F12 media.

A549 cells are a human lung adenocarcinoma cell line from ATCC. The DNApolymerase beta dominant negative A549 cells were provided by Drs AdrianBegg and Conchita Vens (Department of Experimental Therapy, TheNetherlands Cancer Institute, Amsterdam, The Netherlands) [C. Vens, E.Dahmen-Mooren, M. Verwijs-Janssen, W. Blyweert, L. Graversen, H.Bartelink and A. C. Begg (2002) The role of DNA polymerase beta indetermining sensitivity to ionizing radiation in human tumor cells,Nucleic Acids Res. 30:2995-3004]. M059J and M059K human glioma celllines were provided by Dr. Joan Turner (Cross Cancer Institute)[Allalunis-Turner M J, Barron G M, Day R S 3rd, Dobler K D, Mirzayans R.(1993) Isolation of two cell lines from a human malignant gliomaspecimen differing in sensitivity to radiation and chemotherapeuticdrugs. Radiation Research 134:349-354].

The plate was then incubated at 37° C. for 24 hours.3,4-dihydro-5[4-(1-piperindinyl)butoxy]-1(2H)-isoquinoline (DPQ) orA12B4C3 (also referred to as H5 herein, and in the Figures), was thenadded to the plates in varying concentrations. DPQ and A12B4C3 (alsoreferred to as H5 herein, and in the Figures) are PARP and PNKPinhibitors, respectively.

The plate was then incubated at 37° C. for 72 hours. 15 μL of cell titersolution was then added to each of the wells and incubated at 37° C. for4 hours. The cell titer solution contains an MTS compound that isreduced to a formazan product in active cells. The absorbance can thenbe measured at 490 nm using the FluoStar Optima machine.

Results and Discussion

Using the cell proliferation assay, it was determined that human PNKP issynthetically lethal with PARP (FIG. 9) and DNA polymerase β (Pol β)(FIG. 8), but not with DNA-PK (FIG. 10). It was also shown that DNA-PKis synthetically lethal with PARP (FIG. 11).

The results herein are consistent with PARP being synthetically lethalwith proteins involved in DSB repair, PNKP and DNA-PK. Similarly, theSSB repair protein Pol β is synthetically lethal with PNKP. However, thecombination of DNA-PK and PNKP depletion did not show syntheticlethality.

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All publications, patents and patent applications mentioned in thisSpecification are indicative of the level of skill those skilled in theart to which this invention pertains and are herein incorporated byreference to the same extent as if each individual publication patent,or patent application was specifically and individually indicated to beincorporated by reference.

The invention being thus described, it will be obvious that the same maybe varied in many ways. Such variations are not to be regarded as adeparture from the spirit and scope of the invention, and all suchmodification as would be obvious to one skilled in the art are intendedto be included within the scope of the following claims.

1. A kit for increasing the sensitivity of a cancerous cell to aradiation therapy, said kit comprising: a polynucleotidekinase/phosphatase (PNKP) inhibitor or pharmaceutically acceptable saltthereof; and instructions for the use thereof.
 2. The kit of claim 1,wherein said PNKP inhibitor2-(1-hydroxyundecyl)-1-(4-nitrophenylamino)-6-phenyl-6,7a-dihydro-1H-pyrrolo[3,4-b]pyridine-5,7(2H,4aH)-dione(A12B4C3),2-(hydroxy(phenyl)methyl)-1-(4-nitrophenylamino)-6-phenyl-6,7a-dihydro-1H-pyrrolo[3,4-b]pyridine-5,7(2H,4aH)-dione(A1B4C3),2-(hydroxy(3,4,5-trimethoxyphenyl)methyl)-1-(4-nitrophenylamino)-6-phenyl-6,7a-dihydro-1H-pyrrolo[3,4-b]lpyridine-5,7(2H,4aH)-dione(A6B4C3), tert-butyl2-(1-hydroxy-2,2-diphenylethyl)-6-methyl-5,7-dioxo-2,4a,5,6,7,7a-hexahydro-1H-pyrrolo[3,4-b]pyridin-1-ylcarbamate(A26B11C2), or2-(hydroxy(thiophen-2-yl)methyl)-6-methyl-1-(phenylamino)-6,7a-dihydro-1H-pyrrolo[3,4-b]pyridine-5,7(2H,4aH)-dione(A39B1C2).
 3. The kit of claim 1, wherein said radiation therapy isexternal radiation therapy, internal radiation therapy or systemicradiation therapy.
 4. The kit of claim 1, wherein radiation therapy isselected when said patient has cancer of the bladder, brain, breast,cervix, larynx, lung, prostate, vagina, thyroid, pancreas, ovary,breast, uterus, gallbladder, perianal and pelvic regions; colorectalcancers; gynecological cancers; cancer of the small intestine; smallcell lung cancer; head and neck cancer; bronchial cancer; oral cancer;rectal cancer; tracheal cancer; or adult non-Hodgkin lymphoma.
 5. Thekit of claim 1, wherein said cell is from a human, a non-human mammal, arodent, a companion animal or livestock.
 6. A compound orpharmaceutically acceptable salt thereof for increasing the sensitivityof a cancerous cell of a patient to a radiation therapy, said compoundcomprising a polynucleotide kinase/phosphatase (PNKP) inhibitor.
 7. Thecompound of claim 6, wherein said PNKP inhibitor2-(1-hydroxyundecyl)-1-(4-nitrophenylamino)-6-phenyl-6,7a-dihydro-1H-pyrrolo[3,4-b]pyridine-5,7(2H,4aH)-dione (A12B4C3),2-(hydroxy(phenyl)methyl)-1-(4-nitrophenylamino)-6-phenyl-6,7a-dihydro-1H-pyrrolo[3,4-b]pyridine-5,7(2H,4aH)-dione (A1B4C3),2-(hydroxy(3,4,5-trimethoxyphenyl)methyl)-1-(4-nitrophenylamino)-6-phenyl-6,7a-dihydro-1H-pyrrolo[3,4-b]lpyridine-5,7(2H,4aH)-dione(A6B4C3), tert-butyl2-(1-hydroxy-2,2-diphenylethyl)-6-methyl-5,7-dioxo-2,4a,5,6,7,7a-hexahydro-1H-pyrrolo[3,4-b]pyridin-1-ylcarbamate(A26B11C2), or2-(hydroxy(thiophen-2-yl)methyl)-6-methyl-1-(phenylamino)-6,7a-dihydro-1H-pyrrolo[3,4-b]pyridine-5,7(2H,4aH)-dione(A39B1C2).
 8. The compound of claim 6, wherein said radiation therapy isexternal radiation therapy, internal radiation therapy or systemicradiation therapy.
 9. The compound of claim 6, wherein said cancer isbladder cancer, brain cancer, breast cancer, cervix cancer, larynxcancer, lung cancer, prostate cancer, vaginal cancer, thyroid cancer,pancreatic cancer, ovarian cancer, breast cancer, uterine cancer,gallbladder cancer, perianal cancer and pelvic regions; colorectalcancers; gynecological cancers; cancer of the small intestine; smallcell lung cancer; head and neck cancer; bronchial cancer; oral cancer;rectal cancer; tracheal cancer; or adult non-Hodgkin lymphoma.
 10. Thecompound of claim 6, wherein said cell is from a human, a non-humanmammal, a rodent, a companion animal or livestock.
 11. A method of radiosensitizing a cancerous cell in a mammal in need of radiation therapy,comprising: administering to said mammal a polynucleotidekinase/phosphatase (PNKP) inhibitor or pharmaceutically acceptable saltthereof.
 12. The method of claim 11, wherein said PNKP inhibitor2-(1-hydroxyundecyl)-1-(4-nitrophenylamino)-6-phenyl-6,7a-dihydro-1H-pyrrolo[3,4-b]pyridine-5,7(2H,4aH)-dione(A12B4C3),2-(hydroxy(phenyl)methyl)-1-(4-nitrophenylamino)-6-phenyl-6,7a-dihydro-1H-pyrrolo[3,4-b]pyridine-5,7(2H,4aH)-dione (A1B4C3),2-(hydroxy(3,4,5-trimethoxyphenyl)methyl)-1-(4-nitrophenylamino)-6-phenyl-6,7a-dihydro-1H-pyrrolo[3,4-b]lpyridine-5,7(2H,4aH)-dione(A6B4C3), tert-butyl2-(1-hydroxy-2,2-diphenylethyl)-6-methyl-5,7-dioxo-2,4a,5,6,7,7a-hexahydro-1H-pyrrolo[3,4-b]pyridin-1-ylcarbamate(A26B11C2), or2-(hydroxy(thiophen-2-yl)methyl)-6-methyl-1-(phenylamino)-6,7a-dihydro-1H-pyrrolo[3,4-b]pyridine-5,7(2H,4aH)-dione(A39B1C2).
 13. The method of claim 11, wherein said radiation therapy isexternal radiation therapy, internal radiation therapy or systemicradiation therapy.
 14. The method of claim 11, wherein said cancer isbladder cancer, brain cancer, breast cancer, cervix cancer, larynxcancer, lung cancer, prostate cancer, vaginal cancer, thyroid cancer,pancreatic cancer, ovarian cancer, breast cancer, uterine cancer,gallbladder cancer, perianal cancer and pelvic regions; colorectalcancers; gynecological cancers; cancer of the small intestine; smallcell lung cancer; head and neck cancer; bronchial cancer; oral cancer;rectal cancer; tracheal cancer; or adult non-Hodgkin lymphoma.
 15. Themethod of claim 11, wherein said cell is from a human, a non-humanmammal, a rodent, a companion animal or livestock.
 16. An improvedmethod for radiation therapy of a patient with a cancer employing aradiation sensitizer, wherein the improvement comprises treating saidpatient with an effective amount of a polynucleotide kinase/phosphatase(PNKP) inhibitor or pharmaceutically acceptable salt thereof.
 17. Themethod of claim 16, wherein said PNKP inhibitor2-(1-hydroxyundecyl)-1-(4-nitrophenylamino)-6-phenyl-6,7a-dihydro-1H-pyrrolo[3,4-b]pyridine-5,7(2H,4aH)-dione(A12B4C3),2-(hydroxy(phenyl)methyl)-1-(4-nitrophenylamino)-6-phenyl-6,7a-dihydro-1H-pyrrolo[3,4-b]pyridine-5,7(2H,4aH)-dione(A1B4C3),2-(hydroxy(3,4,5-trimethoxyphenyl)methyl)-1-(4-nitrophenylamino)-6-phenyl-6,7a-dihydro-1H-pyrrolo[3,4-b]lpyridine-5,7(2H,4aH)-dione(A6B4C3), tert-butyl2-(1-hydroxy-2,2-diphenylethyl)-6-methyl-5,7-dioxo-2,4a,5,6,7,7a-hexahydro-1H-pyrrolo[3,4-b]pyridin-1-ylcarbamate(A26B11C2), or2-(hydroxy(thiophen-2-yl)methyl)-6-methyl-1-(phenylamino)-6,7a-dihydro-1H-pyrrolo[3,4-b]pyridine-5,7(2H,4aH)-dione(A39B1C2).
 18. The method of claim 16, wherein said radiation therapy isexternal radiation therapy, internal radiation therapy or systemicradiation therapy.
 19. The method of claim 16, wherein said cancer isbladder cancer, brain cancer, breast cancer, cervix cancer, larynxcancer, lung cancer, prostate cancer, vaginal cancer, thyroid cancer,pancreatic cancer, ovarian cancer, breast cancer, uterine cancer,gallbladder cancer, perianal cancer and pelvic regions; colorectalcancers; gynecological cancers; cancer of the small intestine; smallcell lung cancer; head and neck cancer; bronchial cancer; oral cancer;rectal cancer; tracheal cancer; or adult non-Hodgkin lymphoma.
 20. Themethod of claim 16, wherein said cell is from a human, a non-humanmammal, a rodent, a companion animal or livestock.
 21. A method ofinhibiting the phosphatase activity of polynucleotide kinase/phosphatase(PNKP), comprising: contacting a cell with a compound orpharmaceutically acceptable salt thereof selected from A12B4C3, A1B4C3,A6B4C3, A26B11C2 or A39B1C2.